| Division 1 |  Carolina RailHawks | |  Vancouver Whitecaps | | | Wakemed Soccer Park | |
| Division 1 |  Portland Timbers | |  San Jose Earthquakes | | | PGE Park | |
| Division 1 |  Houston Dynamo | |  Kansas City Wizards | | | Robertson Stadium | |
| Division 1 |  Columbus Crew | |  Colorado Rapids | | | Columbus Crew Stadium | |
| Division 2 |  Atlanta United | |  Seattle Sounders | | | ReMax Greater Atlanta Stadium | |
| Division 2 |  Inter Miami | |  Los Angeles FC | | | Chase Stadium | |
| | Division 2 |  Chivas USA | |  D.C. United | | | The Home Depot Centre | |
| Division 2 |  Austin FC | |  Charleston Battery | | | Q2 Stadium | |
| Division 3 |  Carson City Kickers | |  Columbus Rebels | | | The Home Depot Centre | |
| Division 3 |  Mount Tabor Portland | |  Orange County SC | | | Portland Park | |
| Division 3 |  Daniel Island S.A. | |  KC Athletics | | | Blackbaud Stadium | |
| | Division 3 |  Carolina Dynamo | |  De Anza Force S.C. | | | Wakemed Soccer Park | |
| Division 4 |  Colorado Ice White | |  Vancouver FC | | | Dicks Sporting Goods Park | |
| Division 4 |  San Antonio FC | |  Savannah Celtic FC | | | Toyota Field | |
| Division 4 |  Laredo Heat | |  North Sound Seawolves | | | Robertson Stadium | |
| Division 4 |  D.C. Colts | |  Fort Lauderdale FC | | | RFK Stadium | |
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